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VPA reagent is used to measure the valproic acid concentration by a particle enhanced turbidimetric inhibition immunoassay method.1 Particle-bound drug (PBD) binds to valproic acid specific antibody (Ab) resulting in the formation of insoluble aggregates causing turbidity. Non-particle-bound valproic acid in the patient sample competes with the PBD for the antibody binding sites, inhibiting the formation of insoluble aggregates. The rate and amount of particle aggregation is inversely proportional upon the concentration of valproic acid in the sample.
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