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The TSH ELISA test is based on the principle of a solid phase
enzyme-linked immunosorbent assay.9,10 The assay system utilizes
a unique monoclonal antibody directed against a distinct antigenic
determinant on the intact TSH molecule. Mouse monoclonal anti-
TSH antibody is used for solid phase immobilization (on the
microtiter wells). A goat anti-TSH antibody is in the antibodyenzyme
(horseradish peroxidase) conjugate solution. The test
sample is allowed to react simultaneously with the two antibodies,
resulting in the TSH molecules being sandwiched between the solid
phase and enzyme-linked ant
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