|
|
1. Dissolve 56.4 g in 1L of distilled water.
2. Autoclave for 15 minutes at 121 °C.
This convenient 5× concentrate can be stored and diluted as needed to prepare 5 L of 1× M9 minimal salts.
For M9 minimal medium:
1. Aseptically dilute 200 mL of M9 minimal salts, 5× concentrate with 800 mL of sterile water. If necessary, cool to 45-50°C.
2. Aseptically add 20 mL of sterile 1 M glucose and 2 mL of sterile 1 M magnesium sulfate to prepare 1 L of M9 minimal medium.
3. If desired, aseptically add 0.1 mL of 1 M sterile calcium chloride to the M9 minimal medium. The M9 minimal medium may also be supp
|